ABSTRACT
A study on the comparative study of electrolyte patterns in sickle cell disease patients in Owerri was carried out. A total of 60 subjects between the ages of 18 and 40 years were randomly selected. 30 subjects were sickle cell disease patients while 30 subjects who served as the control were not suffering from sickle cell disease. Serum electrolyte were measured using colorimetric method and manufactures standard operating procedure were strictly adhered to. Data were compared between Sickle cell patients and control. Comparism was also done according to patients various age groups. Statistical analyses were done using student independent t-test and anova. Results obtained from the study showed that sickle cell anaemia patients has a significant (P<0.05) decrease in serum sodium (102.93 ± 10.91mEq/L) concentration compared to the control subjects (139.10 ± 5.01mEq/L). Sodium level was lower among the older patient. Potassium concentration of patient (5.53 ±0.37 mEq/L) was significantly in control subjects (4.17 ± 0.49mEq/L). Potassium level was higher among the older patients. Results also gathered from the study showed that sickle cell anaemia resulted in significant (P<0.05) decreased in serum chloride concentration compared to the control subjects. Chloride concentration was 103.07 ± 12.41 and 73.77 ± 8.06 mEqL in control subjects and sickle cell patients respectively. Chloride level was lower among the older patients. Bicarbonate concentration of patient (17.57±2.47mEq/L) was significantly lower when compared to the control subjects (24.17±2.49mEq/L). Bicarbonate was lower among patients of age group 27-33 (17.82± 1.24 mEq/l) and higher in those of age group 18-26 (20.43±2.18 mEq/l). In conclution decrease electrolyte in patients with sickle cell disease probably depends on dehydration accelerated influx and efflux of sodium and potassium ion respectively. The regular measurement of sodium and potassium is necessary in the management of sickle cell patient.
CHAPTER ONE
INTRODUCTION
Sickle cell disease (SCD) is a group of inherited disorders of the beta-hemoglobin chain. Normal hemoglobin has 3 different types of hemoglobin – hemoglobin A, A2, and F. Hemoglobin S in sickle cell disease contains an abnormal beta globin chain encoded by a substitution of valine for glutamic acid on chromosome 11 (Bunn,2007). This is an autosomal recessive disorder. Sickle cell disease refers to a specific genotype in which a person inherits one copy of the HbS gene and another gene coding for a qualitatively or quantitatively abnormal beta globin chain. Sickle cell anemia (HbSS) refers to patients who are homozygous for the HbS gene, while heterozygous forms may pair HbS with genes coding for other types of abnormal hemoglobin such as hemoglobin C, an autosomal recessive mutation which substitutes lysine for glutamic acid. In addition, persons can inherit a combination of HbS and β-thalassemia. The β-thalassemias represent an autosomal recessive disorder with reduced production or absence of β-globin chains resulting in anemia. Other genotype pairs include HbSD, HbSO-Arab and HbSE (Meremiku, 2008).
Sickle hemoglobin in these disorders cause affected red blood cells to polymerize under conditions of low oxygen tension resulting in the characteristic sickle shape. Normal red cells live about 120 days in the blood stream but sickled red cells die after about 10 – 20 days. Because they cannot be replaced fast enough, the blood is chronically short of red blood cells, a condition called anaemia. Aggregation of sickle cells in the microcirculation from inflammation, endothelial abnormalities, and thrombophilia lead to ischemia in end organs and tissues distal to the blockage (Hayes, 2004). Inheritance of sickle cell disease predisposes to four main types of crises: vaso-occlusive, splenic sequestration, aplastic and hemolytic. The morbidity and mortality from these events may be manifested as pain, acute chest syndrome (intrapulmonary sickling), pulmonary hypertension, cardiac abnormalities, cerebrovascular hemorrhage/infarct, splenic or hepatic sequestration, autosplenism, renal disease, liver disease, avascular necrosis of the femoral head, priapism, and life-threatening acute hemolytic or aplastic anemia. Triggers for an acute crisis include hypoxemia, dehydration, acidosis, stress, infection, trauma, hypothermia or in some cases, no identifiable predisposing risk factors (Aldrich and Nagel, 2008).
Electrolytes are substances that become ions in solution and acquire the capacity to conduct electricity. The balance of electrolytes in the body is essential for the normal functioning of the cells and organs. Electrolytes measured by blood testing includes sodium (Na+), Potassium (K+), Chloride (Cl-) and bicarbonate (HCO3-). Electrolytes have normal range values, and complications may arise, if any of the electrolytes are higher or lower than the normal range values. Sodium (Na+) is the major positive ion (cation) in the fluid outside of the cells. In combination with chloride, it forms a salt (NaCl). Sodium (Na+) regulates the amount of total body water and plays a critical role in electrical communication especially in the brain, nervous systems and muscles.
Biochemical abnormalities have been associated with sickle cell disease; however there is paucity of information on the roles of these ions in the pathogenesis and management of sickle cell disease (Oladipo et al., 2005). In sickle cells, an abnormal activation of potassium chloride (K+Cl-) co-transport system was proposed to be involved in cell potassium (K+) loss and dehydration (Vitoux et al., 1989). Deoxygenation of sickle cell is known to increase cation permeability of sodium (Na+), Potassium (K+) and calcium (Ca2+) (Rhoda et al., 1990), At lower Hydrogen ion concentration (pH), urea was able to stimulate potassium – chloride (K-Cl-) loss from sickle cells, leading to cellular dehydration, even in regions of low pulmonary oxygen tension (PO2). Potassium – Chloride (K+ Cl-) co-transport is abnormally active in erythrocytes containing positive charged haemoglobins such as haemoglobin (S) (HbS) On deoxygenation, haemoglobin(S) (HbS) cells exhibit a distinctive solute permeability pathway, P sickle, activated stochastically and partially inhibited by 4,4- disothiocyano 2 – 2 disulfostibene (DIDS) and dipyridamole. It is often referred to as a cation channel although its permeability characteristics remains vague and it molecular identity is unknown (Browning et al., 2007).
- JUSTIFICATION
Sickle cell disease is a public health problem and accounts for a lot of morbidity and mortality in the society. Sickle cell disease is much more prevalent in Africa
and Mediterranean crescent. Although the effects of sickle cell disease (SCD) on general morbidity and mortality have been studied but not much work has been done on the comparative study of the electrolyte pattern in sickle cell disease patients. There is therefore the need to bridge this gap. Therefore this work is done to justify the electrolyte pattern in sickle cell patient in Owerri metropolis.
1.2 AIMS AND OBJECTIVE
(1) To determine the level of the sodium, potassium, bicarbonate and chloride electrolyte in patients with sickle cell disease.
(2) To compare the sodium, potassium, bicarbonate and chloride electrolyte level of patients with sickle cell disease with those of normal individuals.
DISCLAIMER: All project works, files and documents posted on this website, eProjectTopics.com are the property/copyright of their respective owners. They are for research reference/guidance purposes only and some of the works may be crowd-sourced. Please don’t submit someone’s work as your own to avoid plagiarism and its consequences. Use it as a reference/citation/guidance purpose only and not copy the work word for word (verbatim). The paper should be used as a guide or framework for your own paper. The contents of this paper should be able to help you in generating new ideas and thoughts for your own study. eProjectTopics.com is a repository of research works where works are uploaded for research guidance. Our aim of providing this work is to help you eradicate the stress of going from one school library to another in search of research materials. This is a legal service because all tertiary institutions permit their students to read previous works, projects, books, articles, journals or papers while developing their own works. This is where the need for literature review comes in. “What a good artist understands is that nothing comes from nowhere. The paid subscription on eProjectTopics.com is a means by which the website is maintained to support Open Education. If you see your work posted here by any means, and you want it to be removed/credited, please contact us with the web address link to the work. We will reply to and honour every request. Please notice it may take up to 24 – 48 hours to process your request.
